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HiSeq 2500 System User Guide

Table of Contents

Revision History vii

Table of Contents ix

Chapter 1 Overview 1

Introduction 2

HiSeq 2500 Components 3

Start the HiSeq 2500 6

HiSeq 2500 Software 7

Available Disk Space 12

Sample Sheet Overview 13

Sequencing Consumables 14

Additional Resources 16

Chapter 2 Perform a HiSeq v4 Run 17

Introduction 18

HiSeq v4 Sequencing Workflow 19

Run Types for HiSeq v4 Chemistry 20

Enter Run Parameters 21

Load and Prime Reagents 26

Load a Flow Cell 35

Monitor the Run 40

Chapter 3 Perform a TruSeq v3 Run 41

Introduction 42

TruSeq v3 Sequencing Workflow 43

Run Types for TruSeq v3 Chemistry 45

Enter Run Parameters 46

Load and Prime Reagents 51

Load a Flow Cell 59

Monitor the Run 64

Prepare Reagents for Read 2 65

Load Reagents for Read 2 66

Chapter 4 Perform a Rapid Run 69

1 2 3 4 5 6 7 8 9 10 11 12 13 14 ... 153 154

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Directed Electronics equalizer 2500 Uživatelská příručka Strana 9