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Perform a HiSeq v4 Run

Part # 15035786 Rev. D

Run Types for HiSeq v4 Chemistry

The following table shows types of sequencing runs and the number of possible cycles for

each read when using HiSeq v4 chemistry. Use this information as a reference when setting

up the run.

Run Type Read 1

Cycles

Index 1 (i7)

Read Cycles

Index 2 (i5)

Read Cycles

Cycles

Total

Cycles

Single-Read,

Non-Indexed

≤ 126 -- -- -- ≤ 126

Single-Read,

Single-Indexed

≤ 126 6 or 7 ¹

8 ²

-- -- ≤ 133 ¹

≤ 134 ²

Single-Read,

Dual-Indexed

≤ 126 8 8 -- ≤ 142

Paired-End,

Non-Indexed

≤ 126 -- -- ≤ 126 ≤ 252

Paired-End,

Single-Indexed

≤ 126 7 ¹

8 ²

-- ≤ 126 ≤ 259 ¹

≤ 260 ²

Paired-End,

Dual-Indexed

≤ 126 8 7 + 8 ³ ≤ 126 ≤ 275

¹ Number of cycles for single-indexed libraries

² Number of cycles for dual-indexed libraries

³ Index 2 Read of a paired-end dual-indexed run includes 7 additional chemistry-only cycles

1 2 ... 25 26 27 28 29 30 31 32 33 34 35 ... 153 154

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