
Load a Flow Cell
HiSeq 2500 System User Guide
93
Figure 45 Flow Cell Positioned Against Top and Right Guide Pins
A Top Guide Pin
B Right Guide Pins
NOTE
Remove your hand from the flow cell before engaging the vacuum switch to prevent
possible alignment drift over time.
3 Slowly move the flow cell lever to position 1 to engage the vacuum and secure the flow
cell into position. When the flow cell lever is green, the vacuum is engaged.
Figure 46 Flow Cell Lever in Position 1
4 Wait for about 5 seconds, and then slowly move the flow cell lever to position 2. When
the flow cell lever is solid green, the manifolds are in position and the flow cell is
ready for use.
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