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TruSeq v3 Sequencing Workflow

HiSeq 2500 System User Guide

TruSeq v3 Sequencing Workflow

Prepare SBS reagents for Read 1 and indexing reagents. Weigh reagents

after preparation.

For reagent preparation information, see Reagent Preparation Steps on page

42.

Using the control software, enter run parameters.

When prompted, load all SBS reagents for Read 1.

Load SBS reagents for Read 2, except ICB.

Load indexing reagents.

With a used flow cell on the instrument, confirm proper flow.

Prime SBS reagents and measure priming waste.

Load the clustered flow cell for sequencing. Confirm proper flow.

Start the sequencing run.

[Optional] After cycle 1, inspect the first base report, and then continue

The run continues as specified in run parameters.

Prepare paired-end reagents and fresh ICB for Read 2. Weigh reagents

after preparation.

For reagent preparation information, see Reagent Preparation Steps on page

42.

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