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PN 718113AF
28
Laser Induced Fluorescence Detection
Operation
Figure 21 Calibration Wizard – Step 1
Selecting Auto Mode will guide the user through a series of steps that will automatically determine
the appropriate value for the calibration correction factor (CCF).
Select Auto Mode, and click the
Next button. The Calibration Wizard - Step 2 screen appears,
as shown in Figure 22.
NOTE
Manual Calibration is discussed in the section Manual Mode.
Calibration Wizard – Step 2
1
Enter the following calibration parameters: (See Figure 22.)
Detector Channel 1 or 2 (note that the Calibration Wizard must be run separately for each
channel)
Target RFU Value (the value expected for the standard)
Capillary Dimensions – Internal Diameter (in mm), and Total Length (in cm)
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