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Agilent TechnologiesAgilent 1260 Infinity Nanoflow PumpUser Manual

10 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpIntroduction to the PumpHydraulic Path OverviewThe NanoFlow Pump is

100 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesTemperature Out of Range Temperature Out of Range 0: Pump channel ATemperatu

1260 Nanoflow Pump User Manual 101Error Information7Module Error MessagesMotor-Drive PowerThe current drawn by the pump motor exceeded the maximum lim

102 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesEncoder MissingThe optical encoder on the pump motor in the module is missin

1260 Nanoflow Pump User Manual 103Error Information7Module Error MessagesElectro-Magnetic-Proportional-Valve (EMPV) MissingEMPV MissingThe EMPV in the

104 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesServo Restart FailedThe pump motor in the module was unable to move into the

1260 Nanoflow Pump User Manual 105Error Information7Module Error MessagesPump Head MissingThe pump-head end stop in the pump was not found.When the pu

106 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesIndex AdjustmentThe encoder index position in the module is out of adjustmen

1260 Nanoflow Pump User Manual 107Error Information7Module Error MessagesStroke LengthThe distance between the lower piston position and the upper mec

108 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesWait TimeoutWhen running certain tests in the diagnostics mode or other spec

1091260 Nanoflow Pump User ManualAgilent Technologies8Test Functions and CalibrationMicro Mode Pressure Test 110Running the Test from the Agilent Lab

1260 Nanoflow Pump User Manual 11Introduction to the 1260 Infinity Nanoflow Pump1Introduction to the PumpFigure 2 The Hydraulic Path of the NanoFlow P

110 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationMicro Mode Pressure TestMicro Mode Pressure TestDescriptionThis is a fast test to ve

1260 Nanoflow Pump User Manual 111Test Functions and Calibration8Micro Mode Pressure TestMicro Mode Pressure Test ResultsThe test results are evaluate

112 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationNormal Mode Pressure TestNormal Mode Pressure TestDescriptionThe System Pressure Tes

1260 Nanoflow Pump User Manual 113Test Functions and Calibration8Normal Mode Pressure TestPositioning the Blank NutIf a specific component is suspecte

114 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationNormal Mode Pressure TestRunning the Pressure TestRunning the test from the Agilent

1260 Nanoflow Pump User Manual 115Test Functions and Calibration8Normal Mode Pressure TestEvaluating the ResultsThe sum of all leaks between the pump

116 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationLeak TestLeak TestLeak Test DescriptionThe Leak Test is a built-in troubleshooting t

1260 Nanoflow Pump User Manual 117Test Functions and Calibration8Leak TestPlateau 2Piston B2 delivers with a flow rate of 3 µL/min for 30 s.Ramp 5Pist

118 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationLeak TestRunning the Leak TestRunning the test from the Agilent Lab Advisor software

1260 Nanoflow Pump User Manual 119Test Functions and Calibration8Leak TestEvaluating the ResultsDefective or leaky components in the pump head lead to

12 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpIntroduction to the PumpHow Does the Pumping Unit Work?Both pumping

120 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationLeak TestPressure limit not reached but plateaus horizontal or positiveAll plateaus

1260 Nanoflow Pump User Manual 121Test Functions and Calibration8Leak TestFirst plateau negative or unstable, and at least one other plateau positiveS

122 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationLeak TestThird plateau negative or unstable and at least one other plateau positiveP

1260 Nanoflow Pump User Manual 123Test Functions and Calibration8Leak TestFourth plateau negative or unstable and at least one other plateau positiveP

124 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationEMPV TestEMPV TestEMPV Test DescriptionThe test is designed to verify the performanc

1260 Nanoflow Pump User Manual 125Test Functions and Calibration8EMPV CleaningEMPV Cleaning1260 Nanoflow Pump EMPV Cleaning DescriptionDepending on th

126 1260 Nanoflow Pump User Manual8 Test Functions and CalibrationEMPV Cleaning

1271260 Nanoflow Pump User ManualAgilent Technologies9MaintenanceIntroduction to Maintenance 128Warnings and Cautions 129Overview of Maintenance 130Cl

128 1260 Nanoflow Pump User Manual9 MaintenanceIntroduction to MaintenanceIntroduction to MaintenanceThe pump is designed for easy repair. The most fr

1260 Nanoflow Pump User Manual 129Maintenance9Warnings and CautionsWarnings and CautionsWARNINGToxic, flammable and hazardous solvents, samples and re

1260 Nanoflow Pump User Manual 13Introduction to the 1260 Infinity Nanoflow Pump1Introduction to the Pumpcloses the outlet valve, preventing any chamb

130 1260 Nanoflow Pump User Manual9 MaintenanceOverview of MaintenanceOverview of MaintenanceFigure 17 on page 131 shows the main assemblies of the pu

1260 Nanoflow Pump User Manual 131Maintenance9Overview of MaintenanceFigure 17 Overview of Repair Procedures&'()*+,-.&%&'2 Activ

132 1260 Nanoflow Pump User Manual9 MaintenanceCleaning the ModuleCleaning the ModuleThe module case should be kept clean. Cleaning should be done wit

1260 Nanoflow Pump User Manual 133Maintenance9Early Maintenance Feedback (EMF)Early Maintenance Feedback (EMF)Maintenance requires the exchange of com

134 1260 Nanoflow Pump User Manual9 MaintenanceEarly Maintenance Feedback (EMF)EMF CountersThe pump provides a series of EMF counters for the pump hea

1260 Nanoflow Pump User Manual 135Maintenance9Early Maintenance Feedback (EMF)Using the EMF CountersThe user-settable EMF limits for the EMF counters

136 1260 Nanoflow Pump User Manual9 MaintenanceChecking and Cleaning the Solvent Inlet FiltersChecking and Cleaning the Solvent Inlet FiltersWhen If s

1260 Nanoflow Pump User Manual 137Maintenance9Checking and Cleaning the Solvent Inlet FiltersCleaning the Solvent Filters1 Remove the blocked solvent

138 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Active Inlet Valve Cartridge or the Active Inlet ValveExchanging the Active Inlet Valve

1260 Nanoflow Pump User Manual 139Maintenance9Exchanging the Active Inlet Valve Cartridge or the Active Inlet ValveExchanging the Valve Cartridge1 Usi

14 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpIntroduction to the PumpFor pump specifications, see “Performance Sp

140 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Active Inlet Valve Cartridge or the Active Inlet ValveReplacing the Active Inlet Valve B

1260 Nanoflow Pump User Manual 141Maintenance9Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve6 After an exchange of the valve ca

142 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Outlet Valve SieveExchanging the Outlet Valve Sieve1 Using a 1/4 inch wrench disconnect

1260 Nanoflow Pump User Manual 143Maintenance9Exchanging the Outlet Valve Sieve6 Check that the new valve is assembled correctly and that the gold sea

144 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Solvent Selection ValveExchanging the Solvent Selection ValveWhen If leaking internally,

1260 Nanoflow Pump User Manual 145Maintenance9Exchanging the Solvent Selection Valve1 Disconnect the solvent tubes and the active inlet valve connecti

146 1260 Nanoflow Pump User Manual9 MaintenanceRemoving and Disassembling the Pump HeadRemoving and Disassembling the Pump HeadWhen • Exchanging pump

1260 Nanoflow Pump User Manual 147Maintenance9Removing and Disassembling the Pump Head3Place the pump head on a flat surface. Loosen the lock screw (t

148 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Pump Seals and Seal Wear-in ProcedureExchanging the Pump Seals and Seal Wear-in Procedur

1260 Nanoflow Pump User Manual 149Maintenance9Exchanging the Pump Seals and Seal Wear-in Procedure3Clean the pump chambers with lint free cloth. Ensur

1260 Nanoflow Pump User Manual 15Introduction to the 1260 Infinity Nanoflow Pump1Introduction to the PumpHow Does Variable Stroke Volume Work?Due to t

150 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Pump Seals and Seal Wear-in ProcedureSeal Wear-in Procedure1 Place a bottle with 100 ml

1260 Nanoflow Pump User Manual 151Maintenance9Exchanging the PistonsExchanging the PistonsWhen When scratchedTools required • 3-mm hexagonal key• 4-mm

152 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Pistons3Reassemble the pump head assembly (see “Reassembling the Pump Head Assembly” on

1260 Nanoflow Pump User Manual 153Maintenance9Exchanging the Flow SensorExchanging the Flow Sensor1 Turn off the pump.2 Using a 1/4 inch wrench discon

154 1260 Nanoflow Pump User Manual9 MaintenanceReassembling the Pump Head AssemblyReassembling the Pump Head AssemblyTools required • 3-mm hexagonal k

1260 Nanoflow Pump User Manual 155Maintenance9Reassembling the Pump Head Assembly3Carefully insert the pistons into the pump head assembly and press t

156 1260 Nanoflow Pump User Manual9 MaintenanceExchanging the Optional Interface BoardExchanging the Optional Interface Board1 Switch OFF the module a

1260 Nanoflow Pump User Manual 157Maintenance9Replacing Module FirmwareReplacing Module FirmwareTo upgrade/downgrade the module’s firmware carry out t

158 1260 Nanoflow Pump User Manual9 MaintenanceReplacing Module Firmware

1591260 Nanoflow Pump User ManualAgilent Technologies10Parts and Materials for MaintenancePump Housing and Main Assemblies 160Solvent Cabinet and Bott

16 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpEarly Maintenance FeetbackEarly Maintenance FeetbackMaintenance requ

160 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenancePump Housing and Main AssembliesPump Housing and Main AssembliesRepair Parts —

1260 Nanoflow Pump User Manual 161Parts and Materials for Maintenance10Pump Housing and Main AssembliesFigure 24 Overview of Main Assemblies (Front Vi

162 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenancePump Housing and Main AssembliesRepair Parts—Pump Housing and Main Assemblies

1260 Nanoflow Pump User Manual 163Parts and Materials for Maintenance10Solvent Cabinet and Bottle-Head AssemblySolvent Cabinet and Bottle-Head Assembl

164 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenanceHydraulic PathHydraulic PathItem p/n Description1 G1311-60003 Bottle-head asse

1260 Nanoflow Pump User Manual 165Parts and Materials for Maintenance10Hydraulic PathFigure 27 Hydraulic Path(''*(-,&&&'.&a

166 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenancePump-Head AssemblyPump-Head AssemblyThe complete pump head assembly includes i

1260 Nanoflow Pump User Manual 167Parts and Materials for Maintenance10Pump-Head AssemblyFigure 28 Pump-Head Assembly&'()*+,-.&%&&

168 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenanceFlow Sensor AssemblyFlow Sensor AssemblyFigure 29 Flow Sensor Assemblyp/n Desc

1260 Nanoflow Pump User Manual 169Parts and Materials for Maintenance10Nanoflow Pump Accessory KitNanoflow Pump Accessory KitAccessory Kit (Nano Pump)

1260 Nanoflow Pump User Manual 17Introduction to the 1260 Infinity Nanoflow Pump1Instrument LayoutInstrument LayoutThe industrial design of the module

170 1260 Nanoflow Pump User Manual10 Parts and Materials for MaintenanceNanoflow Pump Accessory Kit

1711260 Nanoflow Pump User ManualAgilent Technologies11Identifying CablesCable Overview 172Analog Cables 174Remote Cables 176BCD Cables 179CAN/LAN Cab

172 1260 Nanoflow Pump User Manual11 Identifying CablesCable OverviewCable OverviewAnalog cablesRemote cablesBCD cablesNOTENever use cables other than

1260 Nanoflow Pump User Manual 173Identifying Cables11Cable OverviewCAN cablesLAN cablesExternal Contact CableRS-232 cablesp/n Description5181-1516 CA

174 1260 Nanoflow Pump User Manual11 Identifying CablesAnalog CablesAnalog CablesOne end of these cables provides a BNC connector to be connected to A

1260 Nanoflow Pump User Manual 175Identifying Cables11Analog CablesAgilent Module to BNC ConnectorAgilent Module to General Purposep/n 8120-1840 Pin B

176 1260 Nanoflow Pump User Manual11 Identifying CablesRemote CablesRemote CablesOne end of these cables provides a Agilent Technologies APG (Analytic

1260 Nanoflow Pump User Manual 177Identifying Cables11Remote CablesAgilent Module to 3396 Series III / 3395B IntegratorsAgilent Module to Agilent 3590

178 1260 Nanoflow Pump User Manual11 Identifying CablesRemote CablesAgilent Module to General Purposep/n 01046-60201 Pin Universal Pin Agilent moduleS

1260 Nanoflow Pump User Manual 179Identifying Cables11BCD CablesBCD CablesOne end of these cables provides a 15-pin BCD connector to be connected to t

18 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpElectrical ConnectionsElectrical Connections• The CAN bus is a seria

180 1260 Nanoflow Pump User Manual11 Identifying CablesBCD CablesAgilent Module to 3396 Integratorsp/n 03396-60560 Pin 3396 Pin Agilent moduleSignal N

1260 Nanoflow Pump User Manual 181Identifying Cables11CAN/LAN CablesCAN/LAN CablesBoth ends of this cable provide a modular plug to be connected to Ag

182 1260 Nanoflow Pump User Manual11 Identifying CablesExternal Contact CableExternal Contact CableOne end of this cable provides a 15-pin plug to be

1260 Nanoflow Pump User Manual 183Identifying Cables11Agilent Module to PCAgilent Module to PCp/n DescriptionG1530-60600 RS-232 cable, 2 mRS232-61600

184 1260 Nanoflow Pump User Manual11 Identifying CablesAgilent 1200 Module to PrinterAgilent 1200 Module to Printerp/n Description5181-1529 Cable Prin

1851260 Nanoflow Pump User ManualAgilent Technologies12AppendixGeneral Safety Information 186The Waste Electrical and Electronic Equipment Directive 1

186 1260 Nanoflow Pump User Manual12 AppendixGeneral Safety InformationGeneral Safety Information The following general safety precautions must be obs

1260 Nanoflow Pump User Manual 187Appendix12General Safety InformationMake sure that only fuses with the required rated current and of the specified t

188 1260 Nanoflow Pump User Manual12 AppendixGeneral Safety InformationSafety SymbolsTabl e 24 Safety SymbolsSymbol DescriptionThe apparatus is marked

1260 Nanoflow Pump User Manual 189Appendix12The Waste Electrical and Electronic Equipment DirectiveThe Waste Electrical and Electronic Equipment Direc

1260 Nanoflow Pump User Manual 19Introduction to the 1260 Infinity Nanoflow Pump1Electrical ConnectionsRear View of the ModuleFigure 4 Rear View of th

190 1260 Nanoflow Pump User Manual12 AppendixBatteries InformationBatteries InformationWARNINGLithium batteries may not be disposed-off into the domes

1260 Nanoflow Pump User Manual 191Appendix12Radio InterferenceRadio InterferenceCables supplied by Agilent Technologies are screened to provide optimi

192 1260 Nanoflow Pump User Manual12 AppendixSound EmissionSound EmissionManufacturer’s DeclarationThis statement is provided to comply with the requi

1260 Nanoflow Pump User Manual 193Appendix12Solvent InformationSolvent InformationObserve the following recommendations on the use of solvents.• Brown

194 1260 Nanoflow Pump User Manual12 AppendixAgilent Technologies on InternetAgilent Technologies on InternetFor the latest information on products an

1260 Nanoflow Pump User Manual 195IndexIndex88-bit configuration switchon-board LAN 26without On-Board LAN 28Aactive inlet valve 130, 138, 138Agilen

196 1260 Nanoflow Pump User ManualIndexEMPV test 124EMPV cleaning 81encoder missing 102error messagesfan failed 94error messagescompensation sensor op

1260 Nanoflow Pump User Manual 197Indexremote timeout 91missing pressure reading 98motor-drive power 101Nnon-operating altitude 37non-operating temper

198 1260 Nanoflow Pump User ManualIndexrear view 48status indicator 83stroke length 107stroke volume 15synchronization lost 91system setup and install

1260 Nanoflow Pump User ManualNotices© Agilent Technologies, Inc. 2007, 2008, 2010No part of this manual may be reproduced in any form or by any means

20 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpInterfacesInterfacesThe Agilent 1200 Infinity Series modules provide

© Agilent Technologies 2007, 2008, 2010Printed in Germany 06/2010*G2226-90012**G2226-90012*G2226-90012www.agilent.comAgilent TechnologiesIn This BookT

1260 Nanoflow Pump User Manual 21Introduction to the 1260 Infinity Nanoflow Pump1Interfaces• CAN connectors as interface to other modules• LAN connect

22 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpInterfacesOverview Interfaces CANThe CAN is inter-module communicati

1260 Nanoflow Pump User Manual 23Introduction to the 1260 Infinity Nanoflow Pump1InterfacesThe RS-232C is designed as DCE (data communication equipmen

24 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpInterfacesAPG RemoteThe APG Remote connector may be used in combinat

1260 Nanoflow Pump User Manual 25Introduction to the 1260 Infinity Nanoflow Pump1InterfacesSpecial InterfacesSome modules have module specific interfa

26 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpSetting the 8-bit Configuration Switch (On-Board LAN)Setting the 8-b

1260 Nanoflow Pump User Manual 27Introduction to the 1260 Infinity Nanoflow Pump1Setting the 8-bit Configuration Switch (On-Board LAN)Legend:0 (switch

28 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpSetting the 8-bit Configuration Switch (On-Board LAN)Setting the 8-b

1260 Nanoflow Pump User Manual 29Introduction to the 1260 Infinity Nanoflow Pump1Setting the 8-bit Configuration Switch (On-Board LAN)Communication Se

1260 Nanoflow Pump User Manual 3ContentsContents1 Introduction to the 1260 Infinity Nanoflow Pump 7Introduction to the Pump 8Early Maintenance Feetbac

30 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpSetting the 8-bit Configuration Switch (On-Board LAN)One start bit a

1260 Nanoflow Pump User Manual 31Introduction to the 1260 Infinity Nanoflow Pump1Setting the 8-bit Configuration Switch (On-Board LAN)Special Settings

32 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpSetting the 8-bit Configuration Switch (On-Board LAN)Forced Cold Sta

331260 Nanoflow Pump User ManualAgilent Technologies2Site Requirements and SpecificationsSite Requirements 34Physical Specifications 37Performance Spe

34 1260 Nanoflow Pump User Manual2 Site Requirements and SpecificationsSite RequirementsSite RequirementsA suitable environment is important to ensure

1260 Nanoflow Pump User Manual 35Site Requirements and Specifications2Site RequirementsPower CordsDifferent power cords are offered as options with th

36 1260 Nanoflow Pump User Manual2 Site Requirements and SpecificationsSite RequirementsBench SpaceThe module dimensions and weight (see Table 13 on p

1260 Nanoflow Pump User Manual 37Site Requirements and Specifications2Physical SpecificationsPhysical SpecificationsTabl e 13 Physical SpecificationsT

38 1260 Nanoflow Pump User Manual2 Site Requirements and SpecificationsPerformance SpecificationsPerformance SpecificationsTabl e 14 Performance Speci

1260 Nanoflow Pump User Manual 39Site Requirements and Specifications2Performance SpecificationsAnalog output For pressure monitoring, 2 mV/bar, one o

4 1260 Nanoflow Pump User ManualContents6 Troubleshooting and Diagnostics 79Overview of the Module’s Indicators and Test Functions 80Status Indicators

40 1260 Nanoflow Pump User Manual2 Site Requirements and SpecificationsPerformance Specifications

411260 Nanoflow Pump User ManualAgilent Technologies3Installing the ModuleUnpacking the Module 42Delivery Checklist 42Nanoflow Pump Accessory Kit 43Op

42 1260 Nanoflow Pump User Manual3 Installing the ModuleUnpacking the ModuleUnpacking the ModuleIf the delivery packaging shows signs of external dama

1260 Nanoflow Pump User Manual 43Installing the Module3Unpacking the ModuleNanoflow Pump Accessory KitAccessory Kit (Nano Pump) (p/n G2226-68755)1260

44 1260 Nanoflow Pump User Manual3 Installing the ModuleOptimizing the Stack ConfigurationOptimizing the Stack ConfigurationIf your module is part of

1260 Nanoflow Pump User Manual 45Installing the Module3Optimizing the Stack ConfigurationFigure 8 Recommended Stack Configuration for 1260 (Front View

46 1260 Nanoflow Pump User Manual3 Installing the ModuleOptimizing the Stack ConfigurationFigure 9 Recommended Stack Configuration for 1260 (Rear View

1260 Nanoflow Pump User Manual 47Installing the Module3Optimizing the Stack ConfigurationTwo Stack ConfigurationTo avoid excessive height of the stack

48 1260 Nanoflow Pump User Manual3 Installing the ModuleOptimizing the Stack ConfigurationFigure 11 Recommended Two Stack Configuration for 1260 (Rear

1260 Nanoflow Pump User Manual 49Installing the Module3Installing the PumpInstalling the PumpFor other cables see text below and “Cable Overview” on p

1260 Nanoflow Pump User Manual 5Contents10 Parts and Materials for Maintenance 159Pump Housing and Main Assemblies 160Solvent Cabinet and Bottle-Head

50 1260 Nanoflow Pump User Manual3 Installing the ModuleInstalling the Pump1 Place the module on the bench in a horizontal position.2 Ensure the power

1260 Nanoflow Pump User Manual 51Installing the Module3Installing the Pump5 Connect the required interface cables to the rear of the pump, see “Connec

52 1260 Nanoflow Pump User Manual3 Installing the ModuleConnecting Modules and Control SoftwareConnecting Modules and Control SoftwareConnecting Modul

1260 Nanoflow Pump User Manual 53Installing the Module3Connecting Modules and Control SoftwareConnecting Control Software and/or G4208 A Instant Pilot

54 1260 Nanoflow Pump User Manual3 Installing the ModuleFlow ConnectionsFlow ConnectionsParts required # p/n Description1 Other modules1 G1376-68755 A

1260 Nanoflow Pump User Manual 55Installing the Module3Flow Connections1 Remove the front cover by pressing the snap fasteners on both sides.Figure 14

56 1260 Nanoflow Pump User Manual3 Installing the ModuleFlow Connections7 Purge your system before first use (see “Priming Your System With the Pump”

1260 Nanoflow Pump User Manual 57Installing the Module3Flow Connections1 Capillary EMPV to Nano Flow sensor (4 µL flow sensor) (p/n G1375-87321)2 Cap

58 1260 Nanoflow Pump User Manual3 Installing the ModuleGet the System Ready for the First InjectionGet the System Ready for the First InjectionWhen y

1260 Nanoflow Pump User Manual 59Installing the Module3Get the System Ready for the First Injection7 Repeat steps 4 to 6 for the three remaining solve

6 1260 Nanoflow Pump User ManualContents

60 1260 Nanoflow Pump User Manual3 Installing the ModuleGet the System Ready for the First Injection

611260 Nanoflow Pump User ManualAgilent Technologies4Using the PumpHints For Successfully Using the Pump 62Solvent Information 64Algae Growth in HPLC

62 1260 Nanoflow Pump User Manual4 Using the PumpHints For Successfully Using the PumpHints For Successfully Using the PumpPump Issues• Flush the pump

1260 Nanoflow Pump User Manual 63Using the Pump4Hints For Successfully Using the PumpFused Silica Capillary Issues• When connecting a capillary (espec

64 1260 Nanoflow Pump User Manual4 Using the PumpSolvent InformationSolvent InformationAlways filter solvents through 0.4 µm filters, small particles

1260 Nanoflow Pump User Manual 65Using the Pump4Algae Growth in HPLC SystemsAlgae Growth in HPLC SystemsThe presence of algae in HPLC systems can caus

66 1260 Nanoflow Pump User Manual4 Using the PumpAlgae Growth in HPLC Systems• Algae growth may also be the possible source for failures of the ball v

1260 Nanoflow Pump User Manual 67Using the Pump4Checkout procedure for a G2229A Nano LC SystemCheckout procedure for a G2229A Nano LC SystemUse this p

68 1260 Nanoflow Pump User Manual4 Using the PumpCheckout procedure for a G2229A Nano LC System8 Pump 0.6 µL/min, micro mode, 70 % A (water) / 30 % B

1260 Nanoflow Pump User Manual 69Using the Pump4Checkout procedure for a G2229A Nano LC SystemMethod Parameters Micro High Performance Autosampler• In

71260 Nanoflow Pump User ManualAgilent Technologies1Introduction to the 1260 Infinity Nanoflow PumpIntroduction to the Pump 8Hydraulic Path Overview 1

70 1260 Nanoflow Pump User Manual4 Using the PumpCheckout procedure for a G2229A Nano LC SystemTest Results and EvaluationTypical pressure in bypass m

711260 Nanoflow Pump User ManualAgilent Technologies5Optimizing PerformanceHints for the Micro Vacuum Degasser 72Choosing the Right Pump Seals 73How t

72 1260 Nanoflow Pump User Manual5 Optimizing PerformanceHints for the Micro Vacuum DegasserHints for the Micro Vacuum DegasserIf you are using the va

1260 Nanoflow Pump User Manual 73Optimizing Performance5Choosing the Right Pump SealsChoosing the Right Pump SealsThe standard seal for the pump can b

74 1260 Nanoflow Pump User Manual5 Optimizing PerformanceHow to Choose the Primary FlowHow to Choose the Primary FlowThe primary flow can be set in th

1260 Nanoflow Pump User Manual 75Optimizing Performance5The Standard FilterThe Standard FilterThe standard filter has a volume of typically 100 µL. If

76 1260 Nanoflow Pump User Manual5 Optimizing PerformanceHow to Optimize the Compressibility Compensation SettingHow to Optimize the Compressibility C

1260 Nanoflow Pump User Manual 77Optimizing Performance5How to Optimize the Compressibility Compensation Setting5 Starting with a compressibility sett

78 1260 Nanoflow Pump User Manual5 Optimizing PerformanceHow to Optimize the Compressibility Compensation Setting

791260 Nanoflow Pump User ManualAgilent Technologies6Troubleshooting and DiagnosticsOverview of the Module’s Indicators and Test Functions 80Status In

8 1260 Nanoflow Pump User Manual1 Introduction to the 1260 Infinity Nanoflow PumpIntroduction to the PumpIntroduction to the PumpThe low flow pumps co

80 1260 Nanoflow Pump User Manual6 Troubleshooting and DiagnosticsOverview of the Module’s Indicators and Test FunctionsOverview of the Module’s Indic

1260 Nanoflow Pump User Manual 81Troubleshooting and Diagnostics6Overview of the Module’s Indicators and Test FunctionsLeak Rate TestThe Leak Rate Tes

82 1260 Nanoflow Pump User Manual6 Troubleshooting and DiagnosticsStatus IndicatorsStatus IndicatorsTwo status indicators are located on the front of

1260 Nanoflow Pump User Manual 83Troubleshooting and Diagnostics6Status IndicatorsModule Status IndicatorThe module status indicator indicates one of

84 1260 Nanoflow Pump User Manual6 Troubleshooting and DiagnosticsUser InterfacesUser InterfacesDepending on the User Interface, the available tests v

1260 Nanoflow Pump User Manual 85Troubleshooting and Diagnostics6Agilent Lab Advisor SoftwareAgilent Lab Advisor SoftwareThe Agilent Lab Advisor softw

86 1260 Nanoflow Pump User Manual6 Troubleshooting and DiagnosticsAgilent Lab Advisor Software

871260 Nanoflow Pump User ManualAgilent Technologies7Error InformationWhat Are Error Messages 89General Error Messages 90Timeout 90Shut-Down 90Remote

88 1260 Nanoflow Pump User Manual7 Error InformationAgilent Lab Advisor SoftwareFlow Sensor Missing 103Leak Sensor Missing 103Servo Restart Failed 104

1260 Nanoflow Pump User Manual 89Error Information7What Are Error MessagesWhat Are Error MessagesError messages are displayed in the user interface wh

1260 Nanoflow Pump User Manual 9Introduction to the 1260 Infinity Nanoflow Pump1Introduction to the PumpFigure 1 Overview of the Pump8HBWdVgYEjbeYg^

90 1260 Nanoflow Pump User Manual7 Error InformationGeneral Error MessagesGeneral Error MessagesGeneral error messages are generic to all Agilent seri

1260 Nanoflow Pump User Manual 91Error Information7General Error MessagesRemote TimeoutA not-ready condition is still present on the remote input. Whe

92 1260 Nanoflow Pump User Manual7 Error InformationGeneral Error MessagesLeak Sensor ShortThe leak sensor in the module has failed (short circuit).Th

1260 Nanoflow Pump User Manual 93Error Information7General Error MessagesCompensation Sensor OpenThe ambient-compensation sensor (NTC) on the main boa

94 1260 Nanoflow Pump User Manual7 Error InformationGeneral Error MessagesFan FailedThe cooling fan in the module has failed. The hall sensor on the f

1260 Nanoflow Pump User Manual 95Error Information7Module Error MessagesModule Error MessagesZero Solvent CounterThe error message is triggered if the

96 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesPressure Below Lower LimitThe system pressure has fallen below the lower pres

1260 Nanoflow Pump User Manual 97Error Information7Module Error MessagesValve Failed Valve 0 Failed: valve A1Valve 1 Failed: valve A2Valve 2 Failed: v

98 1260 Nanoflow Pump User Manual7 Error InformationModule Error MessagesMissing Pressure ReadingThe pressure readings read by the pump ADC (analog-di

1260 Nanoflow Pump User Manual 99Error Information7Module Error MessagesValve FuseValve Fuse 0: Channels A1 and A2Valve Fuse 1: Channels B1 and B2One